EMD-8397
In situ structures of the genome and genome-delivery apparatus in ssRNA bacteriophage MS2
EMD-8397
Single-particle3.6 Å
Deposition: 17/09/2016Map released: 07/12/2016
Last modified: 20/11/2024
Name: Enterobacteria phage MS2
Summary:
Name: Enterobacteria phage MS2
Scientific name: Enterobacteria phage MS2
Isolate: SPECIES
Details: The viral stock was obtained from ATCC (ATCC number 15597-B1) and cultured in Escherichia coli strain C-3000 (ATCC 15597).
Scientific name: Enterobacteria phage MS2
Isolate: SPECIES
Details: The viral stock was obtained from ATCC (ATCC number 15597-B1) and cultured in Escherichia coli strain C-3000 (ATCC 15597).
Natural host [1]
Molecular weight
| Experimental | Theoretical | Method |
|---|---|---|
| - | 3 MDa | - |
Virus Shell [1]
| Name | Diameter | triangulation |
|---|---|---|
| Capsid | 270.0 Å | 3 |
Name: capsid shell of Enterobacteria Phage MS2
Details: The capsid of MS2 is composed of 178 copies of the coat protein and one single copy of the maturation protein.
Details: The capsid of MS2 is composed of 178 copies of the coat protein and one single copy of the maturation protein.
Natural source [1]
Organism: Enterobacteria phage MS2
Molecular weight
| Experimental | Theoretical | Method |
|---|---|---|
| - | 2 MDa | - |
Name: coat protein of Enterobacteria Phage MS2
Details: The 178 copies of the coat protein are organized as 89 dimers in a T=3 icosahedral lattice.
Details: The 178 copies of the coat protein are organized as 89 dimers in a T=3 icosahedral lattice.
Natural source [1]
Organism: Enterobacteria phage MS2
Molecular weight
| Experimental | Theoretical | Method |
|---|---|---|
| - | 14 kDa | - |
Name: maturation protein of Enterobacteria Phage MS2
Details: The single copy of maturation protein in the capsid shell of MS2 is located at one of the 2-fold symmetry axes and it replaces a coat protein dimer at this position.
Details: The single copy of maturation protein in the capsid shell of MS2 is located at one of the 2-fold symmetry axes and it replaces a coat protein dimer at this position.
Natural source [1]
Organism: Enterobacteria phage MS2
Molecular weight
| Experimental | Theoretical | Method |
|---|---|---|
| - | 44 kDa | - |
Name: the ssRNA genome of Enterobacteria Phage MS2
Details: The genome of MS2 is a single-stranded RNA with 3569 bases. Our asymmetric cryoEM reconstruction of the MS2 virion shows that its ssRNA genome is well organized and has multiple contacts with the capsid shell via tens of RNA stem-loop structures.
Details: The genome of MS2 is a single-stranded RNA with 3569 bases. Our asymmetric cryoEM reconstruction of the MS2 virion shows that its ssRNA genome is well organized and has multiple contacts with the capsid shell via tens of RNA stem-loop structures.
Natural source [1]
Organism: Enterobacteria phage MS2
Molecular weight
| Experimental | Theoretical | Method |
|---|---|---|
| - | 1 MDa | - |
Name: Capsid protein
Number of copies: 8
Details: The capsid of MS2 contains 178 copies of the coat protein arranged as 89 dimers in a T=3 icosahedral lattice. Structure of the capsid has been solved by crystallography with icosahedral symmetry applied (PDB ID 2MS2, also included as chains A, B, C in this model). Chains D, E, F, G, H are coat proteins that have slightly different structures in the asymmetric cryoEM reconstruction compared to the crystallographic structure. Structures of the other 170 copies of the coat protein are the same with 2MS2, and thus are not included due to the limited number of chain IDs.
UniProtKB PDBe-KB
Number of copies: 8
Details: The capsid of MS2 contains 178 copies of the coat protein arranged as 89 dimers in a T=3 icosahedral lattice. Structure of the capsid has been solved by crystallography with icosahedral symmetry applied (PDB ID 2MS2, also included as chains A, B, C in this model). Chains D, E, F, G, H are coat proteins that have slightly different structures in the asymmetric cryoEM reconstruction compared to the crystallographic structure. Structures of the other 170 copies of the coat protein are the same with 2MS2, and thus are not included due to the limited number of chain IDs.
UniProtKB PDBe-KB
Domains
Natural source
Organism: Enterobacteria phage MS2
Molecular weight
| Experimental | Theoretical | Method |
|---|---|---|
| - | 13 kDa | - |
Name: Maturation protein
Number of copies: 1
Details: The capsid of MS2 contains a single copy of the maturation protein. Our structure shows that it replaces a coat protein dimer at one of the 2-fold icosahedral symmetry axes. Function of the maturation protein is to attach the MS2 virion to the host (E. coli) F-pili and deliver the ssRNA viral genome into the host during infection. of the icosahedral capsid. 178 copies of the coat protein arranged as 89 dimers in a T=3 icosahedral lattice. Structure of the capsid has been solved by crystallography with icosahedral symmetry applied (PDB ID 2MS2, also included as chains A, B, C in this model). Chains D, E, F, G, H are coat proteins that have slightly different structures in the asymmetric cryoEM reconstruction compared to the crystallographic structure. Structures of the other 170 copies of the coat protein are the same with 2MS2, and thus are not included due to the limited number of chain IDs.
UniProtKB PDBe-KB
Number of copies: 1
Details: The capsid of MS2 contains a single copy of the maturation protein. Our structure shows that it replaces a coat protein dimer at one of the 2-fold icosahedral symmetry axes. Function of the maturation protein is to attach the MS2 virion to the host (E. coli) F-pili and deliver the ssRNA viral genome into the host during infection. of the icosahedral capsid. 178 copies of the coat protein arranged as 89 dimers in a T=3 icosahedral lattice. Structure of the capsid has been solved by crystallography with icosahedral symmetry applied (PDB ID 2MS2, also included as chains A, B, C in this model). Chains D, E, F, G, H are coat proteins that have slightly different structures in the asymmetric cryoEM reconstruction compared to the crystallographic structure. Structures of the other 170 copies of the coat protein are the same with 2MS2, and thus are not included due to the limited number of chain IDs.
UniProtKB PDBe-KB
Domains
Natural source
Organism: Enterobacteria phage MS2
Molecular weight
| Experimental | Theoretical | Method |
|---|---|---|
| - | 44 kDa | - |
Name: phage MS2 genome
Number of copies: 1
Number of copies: 1
Natural source
Organism: Enterobacteria phage MS2
Molecular weight
| Experimental | Theoretical | Method |
|---|---|---|
| - | 1 MDa | - |