EMD-8296

Single-particle
7.6 Å
EMD-8296
3D View Gallery
Deposition: 18/07/2016
Map released: 31/08/2016
Last modified: 14/09/2016
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-8296
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Structure of 1x duplex DNA target-bound Cascade/I-C

EMD-8296

Single-particle
7.6 Å
EMD-8296
3D View Gallery
Deposition: 18/07/2016
Map released: 31/08/2016
Last modified: 14/09/2016
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.25 mg/mL
Details: The sample was monodisperse.
Buffer
pH: 7.5
Buffer components [3]:
Name Formula Concentration ChEBI
HEPES - 20.0 mM -
Potassium chloride KCl 100.0 mM
TCEP - 1.0 mM -
Grid
Model: Protochips C-flat 4/2
Material: COPPER
Pretreatment
Type: PLASMA CLEANING
Atmosphere:
Support Film [1]
Material Topology Thickness
CARBON CONTINUOUS -
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal defocus: 2.0 µm - 4.5 µm
Nominal magnification: 27000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Number of grids: 1
Number of real images: 4200
Average exposure time: 6.0 s
Average electron dose per image: 48.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 7.6 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 55000
Software [1]
Name Version Details
RELION 1.4 -
Startup model [1]
Type: OTHER
Details:Negative stain model of complex
⦨ Initial angle assignment
Type: NOT APPLICABLE
Software [1]
Name Version Details
EMAN 2 -
⦩ Final angle assignment
Type: NOT APPLICABLE
Particle selection [1]
Selected Ref. model Method Software Details
203000 - - - -
Final 3D classification
Number of classes: 3
Software [1]
Name Version Details
RELION 1.4 -
CTF correction
Software [1]
Name Version Details
CTFFIND 3 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Structure of 1x duplex DNA target-bound Cascade/I-C
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 160 160 160
Dimensions (Å) 326.4 326.4 326.4
Voxel size (Å) 2.04 2.04 2.04
Contour list
Primary Level Source
True 0.075 AUTHOR