EMD-8243

Single-particle
4.7 Å
EMD-8243
3D View Gallery
Deposition: 10/06/2016
Map released: 13/12/2017
Last modified: 24/10/2018
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-8243
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6bt3 summary report (pdf.gz) Map and 6bt3 FULL report (pdf.gz)
EMICSS annotations (xml)

Near-atomic resolution cryo-EM reconstruction of HPV16 complexed with Fab V5

EMD-8243

Single-particle
4.7 Å
EMD-8243
3D View Gallery
Deposition: 10/06/2016
Map released: 13/12/2017
Last modified: 24/10/2018
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI POLARA 300
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: FEI FALCON II (4k x 4k)
Average electron dose per image: 7.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.7 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 17612
Startup model [1]
Type: OTHER
Details:Auto3dem RMC
⦨ Initial angle assignment
Type: COMMON LINE
⦩ Final angle assignment
Type: COMMON LINE
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: HPV16 complexed with Fab V5
⬡ Geometry
X Y Z
Origin -260 -260 -260
Dimensions (px) 520 520 520
Dimensions (Å) 910.0 910.0 910.0
Voxel size (Å) 1.75 1.75 1.75
Contour list
Primary Level Source
True 1.0 AUTHOR