EMD-8236

Single-particle
5.2 Å
EMD-8236
3D View Gallery
Deposition: 04/06/2016
Map released: 25/10/2017
Last modified: 22/01/2020
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-8236
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5y36 summary report (pdf.gz) Map and 5y36 FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of SpCas9-sgRNA-DNA ternary complex

EMD-8236

Single-particle
5.2 Å
EMD-8236
3D View Gallery
Deposition: 04/06/2016
Map released: 25/10/2017
Last modified: 22/01/2020
Overview 3D View Sample Experiment Validation Additional data Links
Name: SpCas9-sgRNA-target DNA ternay complex
Complex [1]
Name: SpCas9-sgRNA-target DNA ternay complex
Details: S. pyogenes Cas9(D10A, H840A) in complex with a 55-mer target DNA from the fumarylacetoacetate hydrolase (FAH) gene and the corresponding 98-nucleotide sgRNA
Natural source [1]
Organism: Streptococcus pyogenes
Molecular weight
Experimental Theoretical Method
- 225 kDa -