EMD-76394

Single-particle
2.85 Å
EMD-76394
3D View Gallery
Deposition: 02/04/2026
Map released: 29/07/2026
Last modified: 29/07/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-76394

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cryo-EM structure of dCas9 bound to DNA duplex, HNH domain resolved

EMD-76394

Single-particle
2.85 Å
EMD-76394
3D View Gallery
Deposition: 02/04/2026
Map released: 29/07/2026
Last modified: 29/07/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Streptococcus pyogenes M1 GAS
Sample: Catalytically inactive Cas9 complexed with guide RNA and target DNA duplex

Deposition Authors: Grassetti AV , Kinman LF , Davis JH

Engineered Cas9 complexes establish an experimentally grounded benchmark for heterogeneous cryo-EM reconstruction methods
Grassetti AV , Kinman LF , Davis JH
To Be Published