EMD-7610

Single-particle
3.1 Å
EMD-7610
3D View Gallery
Deposition: 21/03/2018
Map released: 18/04/2018
Last modified: 13/03/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-7610
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6ct0 summary report (pdf.gz) Map and 6ct0 FULL report (pdf.gz)
EMICSS annotations (xml)

Atomic Structure of the E2 Inner Core of Human Pyruvate Dehydrogenase Complex

EMD-7610

Single-particle
3.1 Å
EMD-7610
3D View Gallery
Deposition: 21/03/2018
Map released: 18/04/2018
Last modified: 13/03/2024
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.2
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: COUNTING
Average exposure time: 8.0 s
Average electron dose per image: 38.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.1 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 49374
Software [1]
Name Version Details
RELION - -
Startup model [1]
Type: NONE
⦨ Initial angle assignment
Type: NOT APPLICABLE
Software [1]
Name Version Details
RELION - -
⦩ Final angle assignment
Type: NOT APPLICABLE
Software [1]
Name Version Details
RELION - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 3D reconstruction of PDC tE2 after particle polishing
⬡ Geometry
X Y Z
Origin -128 -128 -128
Dimensions (px) 256 256 256
Dimensions (Å) 261.12 261.12 261.12
Voxel size (Å) 1.02 1.02 1.02
Contour list
Primary Level Source
True 2.5 AUTHOR