EMD-7519

Single-particle
8.2 Å
EMD-7519
3D View Gallery
Deposition: 05/03/2018
Map released: 02/05/2018
Last modified: 15/05/2019
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-7519
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

The H/ACA ribonucleoprotein lobe of the cryo-EM structure of substrate-bound human telomerase holoenzyme

EMD-7519

Single-particle
8.2 Å
EMD-7519
3D View Gallery
Deposition: 05/03/2018
Map released: 02/05/2018
Last modified: 15/05/2019
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.0
Grid
Details: Unspecified
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Support Film [1]
Material Topology Thickness
CARBON CONTINUOUS -
Vitrification
Cryogen name: ETHANE-PROPANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Number of real images: 11654
Average electron dose per image: 42.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 8.2 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 36210
Software [1]
Name Version Details
RELION 2.0 -
⦨ Initial angle assignment
Type: OTHER
Details: RELION maximum likelihood
Software [1]
Name Version Details
RELION 2.0 -
⦩ Final angle assignment
Type: OTHER
Details: RELION maximum likelihood
Software [1]
Name Version Details
RELION 2.0 -
Final 3D classification
Software [1]
Name Version Details
RELION 2.0 -
CTF correction
Software [1]
Name Version Details
Gctf - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: The H/ACA RNP lobe of the human telomerase holoenzyme
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 400 400 400
Dimensions (Å) 460.0 460.0 460.0
Voxel size (Å) 1.15 1.15 1.15
Contour list
Primary Level Source
True 0.02 AUTHOR