EMD-7337

Single-particle
4.6 Å
EMD-7337 Deposition: 08/01/2018
Map released: 31/01/2018
Last modified: 11/12/2019
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-7337

Cryo-EM structure of PRC2 bound to cofactors AEBP2 and JARID2 in the Extended Basal state

EMD-7337

Single-particle
4.6 Å
EMD-7337 Deposition: 08/01/2018
Map released: 31/01/2018
Last modified: 11/12/2019
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.9
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: SPOT SCAN
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Average electron dose per image: 80.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 4.6 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 78264
Initial angle assignment
Type: OTHER
Final angle assignment
Type: OTHER
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Sharpened map (EB state)
Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 144 144 144
Dimensions (Å) 299.52 299.52 299.52
Voxel size (Å) 2.08 2.08 2.08
Contour list
Primary Level Source
True 0.08 AUTHOR