EMD-72204
Cryo-EM structure of translating Escherichia coli 70S ribosome bound to mRNA, P-site QKF-peptidyl-tRNAPhe, glycyl-tRNAGly in A/T conformation, EF-Tu-GDP, and bottromycin at 2.01A resolution
EMD-72204
Single-particle2.01 Å
Deposition: 19/08/2025Map released: 26/08/2026
Last modified: 23/09/2026
Sample Organism:
Escherichia coli
Sample: Escherichia coli 70S ribosome bound to mRNA, P-site QKF-peptidyl-tRNAPhe, glycyl-tRNAGly in A/T conformation, EF-Tu-GDP, and bottromycin
Fitted models: 9q3q
Deposition Authors: Travin DY, Basu RS
,
Paranjpe M
,
Klepacki D,
Zhurakovskaya AI
,
Vazquez-Laslop N
,
Mankin AS,
Polikanov YS
,
Gagnon MG
Sample: Escherichia coli 70S ribosome bound to mRNA, P-site QKF-peptidyl-tRNAPhe, glycyl-tRNAGly in A/T conformation, EF-Tu-GDP, and bottromycin
Fitted models: 9q3q
Deposition Authors: Travin DY, Basu RS
,
Paranjpe M
,
Klepacki D,
Zhurakovskaya AI
,
Vazquez-Laslop N
,
Mankin AS,
Polikanov YS
,
Gagnon MG
Sequence-specific trapping of EF-Tu/glycyl-tRNA complex on the ribosome by bottromycin
Travin DY,
Basu RS
,
Paranjpe M
,
Klepacki D,
Zhurakovskaya AI
,
Vazquez-Laslop N
,
Mankin AS,
Polikanov YS
,
Gagnon MG
To Be Published
,
Paranjpe M
,
Klepacki D,
Zhurakovskaya AI
,
Vazquez-Laslop N
,
Mankin AS,
Polikanov YS
,
Gagnon MG
To Be Published
Grant Support:
- National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS): R01 GM132302 (United States)
- National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS): R35 GM151957 (United States)
- National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS): R01 GM136936 (United States)
- National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS): R35 GM158272 (United States)
- National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS): R35 GM127134 (United States)
- National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID): R01 AI162961 (United States)
- Welch Foundation: H-2032-20230405 (United States)
