EMD-69299

Single-particle
2.39 Å
EMD-69299
3D View Gallery
Deposition: 21/02/2026
Map released: 02/09/2026
Last modified: 02/09/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-69299

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-23vw summary report (pdf.gz) Map-23vw FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cryo-EM structure of the TfCas12m3-crRNA-tgDNA ternary complex

EMD-69299

Single-particle
2.39 Å
EMD-69299
3D View Gallery
Deposition: 21/02/2026
Map released: 02/09/2026
Last modified: 02/09/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Thermanaerosceptrum fracticalcis, DNA molecule, Lachnospiraceae bacterium ND2006
Sample: TfCas12m3-crRNA-target DNA
Fitted models: 23vw

Deposition Authors: Morinaga H, Omura SN, Nureki O

Intact RuvC catalytic site and bipartite ssDNA kinking are required for Cas12m nuclease activation
Morinaga H, Omura SN, Alfonse L, Ornstein A, Kobayashi TA, Onishi K, Makarova KS, Shmakov SA, Munoz G, Garrity AJ, DiTommaso T, Koonin EV, Maben Z, Nureki O
To Be Published