EMD-69298

Single-particle
2.84 Å
EMD-69298
3D View Gallery
Deposition: 21/02/2026
Map released: 02/09/2026
Last modified: 02/09/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-69298

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-23vv summary report (pdf.gz) Map-23vv FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cryo-EM structure of the UnCas12m4a-crRNA-tgDNA ternary complex in the NTS-cleaving state

EMD-69298

Single-particle
2.84 Å
EMD-69298
3D View Gallery
Deposition: 21/02/2026
Map released: 02/09/2026
Last modified: 02/09/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: bacterium, Lachnospiraceae bacterium ND2006
Sample: UnCas12m4a-crRNA-target DNA (NTS-cleaving state)
Fitted models: 23vv

Deposition Authors: Morinaga H, Omura SN, Nureki O

Intact RuvC catalytic site and bipartite ssDNA kinking are required for Cas12m nuclease activation
Morinaga H, Omura SN, Alfonse L, Ornstein A, Kobayashi TA, Onishi K, Makarova KS, Shmakov SA, Munoz G, Garrity AJ, DiTommaso T, Koonin EV, Maben Z, Nureki O
To Be Published