EMD-65909

Single-particle
3.72 Å
EMD-65909
3D View Gallery
Deposition: 19/08/2025
Map released: 26/08/2026
Last modified: 26/08/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-65909

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9wdx summary report (pdf.gz) Map-9wdx FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Structure of SpCas9 with engineered disulfide bonds (R1210C/V1280C and L1119C/P1128C)

EMD-65909

Single-particle
3.72 Å
EMD-65909
3D View Gallery
Deposition: 19/08/2025
Map released: 26/08/2026
Last modified: 26/08/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Streptococcus pyogenes serotype M1
Sample: SpCas9 with engineered disulfide bonds (R1210C/V1280C and L1119C/P1128C)
Fitted models: 9wdx

Deposition Authors: Teo ZT, Ravi LRV, Jia X, Xue B, Qu K, Yew WS

Engineering Enhanced Thermostability and High-Temperature Activity in SpCas9 through Strategic C-Terminal Domain Disulfide Bond Incorporation
Teo ZT, Hartono YD, Xue B, Jia X, Ravi LRV, Qu K, Carstens C-P, Bruhn L, Yew WS
To Be Published
Grant Support: