EMD-65732

Single-particle
3.91 Å
EMD-65732
3D View Gallery
Deposition: 06/08/2025
Map released: 31/12/2025
Last modified: 06/05/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-65732

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9w7t summary report (pdf.gz) Map-9w7t FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class B

EMD-65732

Single-particle
3.91 Å
EMD-65732
3D View Gallery
Deposition: 06/08/2025
Map released: 31/12/2025
Last modified: 06/05/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Streptococcus pyogenes, Streptococcus pyogenes serotype M1
Sample: SuperFi Cas9 - 20nt sgRNA - DNA ternary complex Class B
Fitted models: 9w7t

Deposition Authors: Zheng R, Ma LJ
Improving the efficiency of high-fidelity Cas9 by enhancing PAM-distal interactions.
Zheng R, Lu Z, Wei R, Shin YC, Du J, Zhang Q, Li J, Wang X, Wei Y, Liu B, Chen Y, Ding L, Zhang H, Chen H, Huang J, Ma L
(2026) Nat Struct Mol Biol , 33 , 590 - 602
PUBMED: 41851507
DOI: doi:10.1038/s41594-026-01753-3
ISSN: 1545-9985
Grant Support: