EMD-6500

Single-particle
15.0 Å
EMD-6500
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Deposition: 02/11/2015
Map released: 22/06/2016
Last modified: 22/06/2016
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-6500
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Negative stain EM reconstruction of HIV-1 Env AMC008 SOSIP.v4 in complex with Fabs 35O22 and PGV04

EMD-6500

Single-particle
15.0 Å
EMD-6500
3D View Gallery
Deposition: 02/11/2015
Map released: 22/06/2016
Last modified: 22/06/2016
Overview 3D View Sample Experiment Validation Additional data Links
Name: HIV-1 Env AMC008 SOSIP.v4 in complex with broadly neutralizing antibody Fabs 35O22 and PGV04
Sample [1]
Name: HIV-1 Env AMC008 SOSIP.v4 in complex with broadly neutralizing antibody Fabs 35O22 and PGV04
Number of unique components: 3
Oligomeric state: 3 of each Fab bound to a trimer of HIV-1 Env SOSIP.v4
Details: All components were purified by size-exclusion chromatography prior to complex formation.
Molecular weight
Experimental Theoretical Method
- 720 kDa Estimation based on average molecular weight of Fab and average mass of SOSIP trimer including glycan mass
Protein [3]
Recombinant expression
Name: HIV-1 AMC008 Env SOSIP.v4 (HIV-1 gp140)
Number of copies: 1
Oligomeric state: Trimer
Natural source
Organism: Human immunodeficiency virus 1
Strain: AMC008
Molecular weight
Experimental Theoretical Method
- 420 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Homo sapiens - HEK293F -
Recombinant expression
Name: Anti-HIV-1 antibody PGV04 Fab
Number of copies: 3
Oligomeric state: Heterodimer of light and heavy chain
Natural source
Organism: Homo sapiens (Human)
Molecular weight
Experimental Theoretical Method
- 50 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Homo sapiens - HEK293F -
Recombinant expression
Name: Anti-HIV-1 antibody 35O22 Fab
Number of copies: 3
Oligomeric state: Heterodimer of light and heavy chain
Natural source
Organism: Homo sapiens (Human)
Molecular weight
Experimental Theoretical Method
- 50 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Homo sapiens - HEK293F -