EMD-6467

Single-particle
14.9 Å
EMD-6467
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Deposition: 13/09/2015
Map released: 13/01/2016
Last modified: 13/01/2016
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-6467
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EMICSS annotations (xml)

Cryo-EM of CHIKV VLP in complex with Fab fragment of neutralizing antibody C9

EMD-6467

Single-particle
14.9 Å
EMD-6467
3D View Gallery
Deposition: 13/09/2015
Map released: 13/01/2016
Last modified: 13/01/2016
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 2 mg/mL
Buffer
pH: 7.4
Details: 20 mM HEPES, 150 mM NaCl
Grid
Details: Quantifoil copper grids (R2/1, 200 mesh)
Vitrification
Cryogen name: ETHANE
Chamber humidity: 90%
Chamber temperature: 120 K
Instrument: FEI VITROBOT MARK III
Method: Blot for 2.5 seconds before plunging.
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 1.0 µm - 3.0 µm
Nominal magnification: 80000.0
Specimen holder model: GATAN LIQUID NITROGEN
Alignment procedure: LEGACY (Astigmatism: Objective lens astigmatism was corrected at 150,000 times magnification., Electron beam tilt params: )
Temperature
Average: 100 K
Image Recording [1]
Detector category: CCD
Detector model: TVIPS TEMCAM-F816 (8k x 8k)
Sampling interval: 15.6 µm
Number of real images: 350
Average electron dose per image: 18 e/Å2
Bits per pixel: 32.0
Image processing [1]
Details: Particle images were automatically boxed out using the program ethan.
Final reconstruction
Resolution: 14.9 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 5879
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: I
Software [1]
Name Version Details
Relion_1.3 - -
CTF correction
Details: Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of CHIKV VLP in complex with Fab fragment of neutralizing antibody C9
Details: ::::EMDATABANK.org::::EMD-6467::::
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 360 360 360
Dimensions (Å) 1130.4 1130.4 1130.4
Voxel size (Å) 3.14 3.14 3.14
Contour list
Primary Level Source
True 0.021 AUTHOR