EMD-64254

Single-particle
2.77 Å
EMD-64254
3D View Gallery
Deposition: 18/04/2025
Map released: 22/10/2025
Last modified: 10/06/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-64254

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9ukt summary report (pdf.gz) Map-9ukt FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

CryoEM structure of the T.thermophilus transcription initiation complex bound to Gp4A-C-U and CTP, -1 dC in the template DNA strand

EMD-64254

Single-particle
2.77 Å
EMD-64254
3D View Gallery
Deposition: 18/04/2025
Map released: 22/10/2025
Last modified: 10/06/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Thermus thermophilus HB8
Sample: T.thermophilus transcription initiation complex bound to Gp4A-C-U (-1dC)
Fitted models: 9ukt

Deposition Authors: Duan W, Kaushik A, Serganov A
Molecular basis for noncanonical transcription initiation from Np 4 A alarmones.
Duan W, Kaushik A, Unarta IC, Wu Y, Liu MMJ, Weaver JW, Wang B, Rice WJ , Luciano DJ, Belasco JG , Huang X , Nudler E , Serganov A
(2026) Nat Chem Biol , 22 , 906 - 916
PUBMED: 41094128
DOI: doi:10.1038/s41589-025-02044-6
ISSN: 1552-4469