EMD-63767
Cryo-EM structure of wild-type SaCas9-guide RNA-mismatched target DNA complex
EMD-63767
Single-particle3.01 Å
Deposition: 15/03/2025Map released: 18/03/2026
Last modified: 29/04/2026
Sample Organism:
Staphylococcus aureus
Sample: Wild-type SaCas9-sgRNA-mismatched target DNA ternary complex
Fitted models: 9mb6
Deposition Authors: Omura SN
,
Nakagawa R ,
Yamashita K ,
Nishimasu H ,
Nureki O
Sample: Wild-type SaCas9-sgRNA-mismatched target DNA ternary complex
Fitted models: 9mb6
Deposition Authors: Omura SN
,
Nakagawa R ,
Yamashita K ,
Nishimasu H ,
Nureki O
Engineering a compact high-fidelity Staphylococcus aureus Cas9 variant with broader targeting range and mechanistic insights into its activation.
Omura SN ,
Nakagawa R ,
Kajimoto S,
Okazaki S,
Ishiguro S,
Mori H,
Onishi K,
Kashiwakura Y,
Hiramoto T,
Horinaka K,
Tanaka M,
Hirano H,
Jividen K,
Yamashita K ,
Tsai SQ,
Yachie N,
Ohmori T,
Nishimasu H ,
Nureki O
(2026) Nat Commun , 17
,
Nakagawa R ,
Kajimoto S,
Okazaki S,
Ishiguro S,
Mori H,
Onishi K,
Kashiwakura Y,
Hiramoto T,
Horinaka K,
Tanaka M,
Hirano H,
Jividen K,
Yamashita K ,
Tsai SQ,
Yachie N,
Ohmori T,
Nishimasu H ,
Nureki O
(2026) Nat Commun , 17
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