EMD-6319

Subtomogram averaging
11.3 Å
EMD-6319
3D View Gallery
Deposition: 17/04/2015
Map released: 09/12/2015
Last modified: 09/12/2015
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-6319
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 3ja6 summary report (pdf.gz) Map and 3ja6 FULL report (pdf.gz)
EMICSS annotations (xml)

Structure of bacterial chemotaxis signaling CheA2-trimer core complex by cryo-electron tomography and subvolume averaging

EMD-6319

Subtomogram averaging
11.3 Å
EMD-6319
3D View Gallery
Deposition: 17/04/2015
Map released: 09/12/2015
Last modified: 09/12/2015
Overview 3D View Sample Experiment Validation Additional data Links
Name: tarCF CheA CheW
Sample [1]
Name: tarCF CheA CheW
Number of unique components: 3
Oligomeric state: Trimer of (CheA dimer, dimer of tarCF trimers of dimers, 2 CheW subunits)
Molecular weight
Experimental Theoretical Method
- 1 MDa -
Protein [3]
Recombinant expression
Name: Chemotaxis protein CheA (Bacterial Chemotaxis Histidine Kinase CheA)
Number of copies: 6
Oligomeric state: Dimer
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Escherichia coli
Cellular location: Inner Membrane
Molecular weight
Experimental Theoretical Method
- 71 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli RP3098 - pKJ9
Recombinant expression
Name: Chemotaxis protein CheW
Number of copies: 2
Oligomeric state: monomeric
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Escherichia coli
Cellular location: Inner Membrane
Molecular weight
Experimental Theoretical Method
- 18 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli RP3098 - PPA770
Recombinant expression
Name: Methyl-accepting chemotaxis protein II (tarCF)
Number of copies: 6
Oligomeric state: trimer of dimers
Details: Cytoplasmic fragment of wild-type aspartate receptor
UniProtKB PDBe-KB AlphaFold DB
Natural source
Organism: Escherichia coli
Cellular location: Inner Membrane
Molecular weight
Experimental Theoretical Method
- 31 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli RP3098 - pHTCF