EMD-62471

Single-particle
2.7 Å
EMD-62471
3D View Gallery
Deposition: 20/11/2024
Map released: 30/07/2025
Last modified: 30/07/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-62471

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9ko9 summary report (pdf.gz) Map-9ko9 FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cryo-EM structure of PsCas9-sgRNA binary complex

EMD-62471

Single-particle
2.7 Å
EMD-62471
3D View Gallery
Deposition: 20/11/2024
Map released: 30/07/2025
Last modified: 30/07/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Parasutterella secunda
Sample: type II CRISPR RNA-guided endonuclease Cas9
Fitted models: 9ko9

Deposition Authors: Shen PP, Lu XY, Huang J-W, Chen C-C, Gut R-T
Structural and Mechanistic Investigations of a High-Fidelity Cas9 from Parasutterella secunda
Shen P, Lu X, Wang C, Huang JW, Min J, Luo J, Sun Y, Chen CC, Guo RT
(2025) ACS Catal , 15 , 10109 - 10118
DOI: doi:10.1021/acscatal.5c02993
ISSN: 2155-5435