EMD-61853

Single-particle
3.46 Å
EMD-61853
3D View Gallery
Deposition: 10/10/2024
Map released: 17/09/2025
Last modified: 28/01/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-61853

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9jwk summary report (pdf.gz) Map-9jwk FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cryo-EM structure of FrCas9 in complex with sgRNA and 26-nt TS and 4-nt NTS substrates

EMD-61853

Single-particle
3.46 Å
EMD-61853
3D View Gallery
Deposition: 10/10/2024
Map released: 17/09/2025
Last modified: 28/01/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Faecalibaculum rodentium, Homo sapiens
Sample: Ternary complex of FrCas9-sgRNA bound to 26-nt TS and 4-nt NTS
Fitted models: 9jwk

Deposition Authors: Chen SD, Yang M, Liu SQ
Structural and functional bases of F. rodentium Cas9 provide insights into CRISPR-Cas protein engineering.
Yang M, Liu S, Chen G, Liu X, Sun D, Zhang J, Wang Y, Chen S, Tian R, Hu Z
(2026) Cell Genom , 6 , 101039 - 101039
PUBMED: 41106392
DOI: doi:10.1016/j.xgen.2025.101039
ISSN: 2666-979X
Grant Support: