EMD-6058

Single-particle
19.0 Å
EMD-6058
3D View Gallery
Deposition: 27/08/2014
Map released: 05/11/2014
Last modified: 17/12/2014
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-6058
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EMICSS annotations (xml)

Negative stain EM structure of the yeast dynein motor domain in the presence of ATP

EMD-6058

Single-particle
19.0 Å
EMD-6058
3D View Gallery
Deposition: 27/08/2014
Map released: 05/11/2014
Last modified: 17/12/2014
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.02 mg/mL
Buffer
pH: 8.0
Details: 50 mM Tris, pH 8.0, 200 mM sodium chloride, 1 mM EGTA, 2 mM magnesium acetate, 1 mM DTT
Staining
Type: NEGATIVE
Details: Samples were applied to freshly glow discharged carbon coated grids and blotted off. Immediately after blotting, a 2% uranyl formate solution was applied for staining and blotted off. The stain was applied five times per sample. Samples were allowed to air dry before imaging.
Grid
Details: Cu 400 mesh
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 1.2 µm - 3.5 µm
Nominal magnification: 80000.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Image Recording [1]
Detector category: CCD
Detector model: TVIPS TEMCAM-F416 (4k x 4k)
Number of real images: 611
Average electron dose per image: 54 e/Å2
Image processing [1]
Final reconstruction
Resolution: 19.0 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 924
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
Relion - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Reconstruction of yeast dynein motor domain in the presence of 5 mM ATP
Details: ::::EMDATABANK.org::::EMD-6058::::
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 64 64 64
Dimensions (Å) 272.64 272.64 272.64
Voxel size (Å) 4.26 4.26 4.26
Contour list
Primary Level Source
True 0.05 EMDB