EMD-5813

Single-particle
30.0 Å
EMD-5813
3D View Gallery
Deposition: 02/12/2013
Map released: 15/01/2014
Last modified: 15/01/2014
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-5813
Download
3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Negative-stain electron microscopy reconstruction of Tetrahymena telomerase (Teb1-f, conformation VIII)

EMD-5813

Single-particle
30.0 Å
EMD-5813
3D View Gallery
Deposition: 02/12/2013
Map released: 15/01/2014
Last modified: 15/01/2014
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 8.0
Details: 20 mM HEPES, 50 mM NaCl, 1 mM MgCl2, 1 mM TCEP
Staining
Type: NEGATIVE
Details: 0.8% uranyl formate
Grid
Details: 200 mesh grid with thin carbon support
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal CS: 2.0 mm
Nominal defocus: 1.2 µm - 3.7 µm
Nominal magnification: 70000.0
Calibrated magnification: 68027.0
Specimen holder model: SIDE ENTRY, EUCENTRIC
Minimum tilt angle: 65
Maximum tilt angle: 65
Temperature
Average: 298 K
Image Recording [1]
Detector category: CCD
Detector model: TVIPS TEMCAM-F415 (4k x 4k)
Number of real images: 934
Average electron dose per image: 40 e/Å2
Bits per pixel: 16.0
Image processing [1]
Details: The RCT 3D reconstruction was done using SPIDER.
Final reconstruction
Resolution: 30.0 Å ( BY AUTHOR)
Resolution method: OTHER
Number of images used: 460
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: C1
Software [1]
Name Version Details
SPIDER - -
CTF correction
Details: Each particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: 3D reconstruction of Tetrahymena telomerase (Teb1-f, conformation VIII) using Random Conical Tilt (RCT) method
Details: ::::EMDATABANK.org::::EMD-5813::::
⬡ Geometry
X Y Z
Origin -48 -48 -48
Dimensions (px) 96 96 96
Dimensions (Å) 423.36 423.36 423.36
Voxel size (Å) 4.41 4.41 4.41
Contour list
Primary Level Source
True 0.026 AUTHOR