EMD-56971
Molecular basis of ZPD homopolymerization: cryo-EM structure of a native vertebrate egg coat filament
EMD-56971
Single-particle4.6 Å
Deposition: 02/03/2026Map released: 18/03/2026
Last modified: 22/04/2026
Sample Organism:
Gallus gallus
Sample: Native chicken ZPD homopolymeric filament
Fitted models: 28yj
Deposition Authors: Banjara S, Okumura H
,
Jovine L
Sample: Native chicken ZPD homopolymeric filament
Fitted models: 28yj
Deposition Authors: Banjara S, Okumura H
,
Jovine L
AlphaFold as a prior: experimental structure determination conditioned on a pretrained neural network.
Fadini A ,
Li M ,
McCoy AJ,
Banjara S,
Okumura H ,
Napier E,
Fontana P ,
Khan AR,
Jovine L ,
Terwilliger TC ,
Read RJ ,
Hekstra DR ,
AlQuraishi M
(2026) Nat Methods , 23 , 785 - 795
,
Li M ,
McCoy AJ,
Banjara S,
Okumura H ,
Napier E,
Fontana P ,
Khan AR,
Jovine L ,
Terwilliger TC ,
Read RJ ,
Hekstra DR ,
AlQuraishi M
(2026) Nat Methods , 23 , 785 - 795
Secondary citations:
- Okumura H, Kohno Y, Iwata Y, Mori H, Aoki N, Sato C, Kitajima K, Nadano D & Matsuda T. (2004) A newly identified zona pellucida glycoprotein, ZPD, and dimeric ZP1 of chicken egg envelope are involved in sperm activation on sperm-egg interaction. Biochem J, 384, 191 - 199
- Nishio S, Okumura H & Matsuda T. (2018) Egg-Coat and Zona Pellucida Proteins of Chicken as a Typical Species of Aves. 130, 307 - 329
- Stsiapanava A, Xu C, Brunati M, Zamora-Caballero S, Schaeffer C, Bokhove M, Han L, Hebert H, Carroni M, Yasumasu S, Rampoldi L, Wu B & Jovine L. (2020) Cryo-EM structure of native human uromodulin, a zona pellucida module polymer. EMBO J, 39, e106807 - e106807
- Nishio S, Emori C, Wiseman B, Fahrenkamp D, Dioguardi E, Zamora-Caballero S, Bokhove M, Han L, Stsiapanava A, Algarra B, Lu Y, Kodani M, Bainbridge RE, Komondor KM, Carlson AE, Landreh M, de Sanctis D, Yasumasu S, Ikawa M & Jovine L. (2024) ZP2 cleavage blocks polyspermy by modulating the architecture of the egg coat. Cell, 187, 1440 - 1459.e24
Grant Support:
- Swedish Research Council: 2020-04936 (Sweden)
- Swedish Research Council: 2024-05336 (Sweden)
- Knut and Alice Wallenberg Foundation: 2018.0042 (Sweden)