EMD-5389

Single-particle
25.0 Å
EMD-5389
3D View Gallery
Deposition: 06/02/2012
Map released: 03/04/2013
Last modified: 10/04/2013
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-5389
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An RNA Degradation Machine Sculpted by Ro Autoantigen and Noncoding RNA

EMD-5389

Single-particle
25.0 Å
EMD-5389
3D View Gallery
Deposition: 06/02/2012
Map released: 03/04/2013
Last modified: 10/04/2013
Overview 3D View Sample Experiment Validation Additional data Links
Name: Rsr/Y RNA/PNPase complex
Sample [1]
Name: Rsr/Y RNA/PNPase complex
Number of unique components: 3
Oligomeric state: one Rsr-Y RNA complex binds to one PNPase trimer
Details: The sample was monodisperse and homogeneous.
Molecular weight
Experimental Theoretical Method
375 kDa 365 kDa glycerol gradient
Protein [2]
Recombinant expression
Name: Ro sixty-related (Rsr, Ro 60 kDa autoantigen)
Number of copies: 1
Oligomeric state: monomer
Details: His-tag Rsr
Natural source
Organism: Deinococcus radiodurans
Molecular weight
Experimental Theoretical Method
- 62 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - RSFDuet-1
Recombinant expression
Name: polynucleotide phosphorylase (PNPase)
Number of copies: 3
Oligomeric state: Trimer
Details: Strep-tag PNPase
Natural source
Organism: Deinococcus radiodurans
Molecular weight
Experimental Theoretical Method
- 262 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli - - RSFDuet-1
RNA [1]
OTHER OTHER
Name: Y RNA
Details: Y RNA generated with correct 3' end using hammerhead ribozyme
Natural source
Organism: Deinococcus radiodurans
Molecular weight
Experimental Theoretical Method
- 42 kDa -