EMD-53854
Cryo-EM structure of the human mitochondrial RNA polymerase transcription initiation complex (POLRMT/TFAM/TFB2M/DNA/RNA) with a slipped 3-mer RNA (pppGpGpA) and GTP poised for catalysis (slipped pre-IC4)
EMD-53854
Single-particle3.1 Å
Deposition: 19/05/2025Map released: 30/07/2025
Last modified: 03/09/2025
Sample Organism:
Homo sapiens,
synthetic construct
Sample: human mitochondrial RNA polymerase transcription initiation complex (POLRMT/TFAM/TFB2M/DNA/RNA) with a slipped 3-mer RNA (pppGpGpA) and GTP poised for catalysis (slipped pre-IC4)
Fitted models: 9r96
Deposition Authors: Goovaerts Q
,
Shen J,
Ajjugal Y ,
De Wijngaert B,
Patel SS ,
Das K
Sample: human mitochondrial RNA polymerase transcription initiation complex (POLRMT/TFAM/TFB2M/DNA/RNA) with a slipped 3-mer RNA (pppGpGpA) and GTP poised for catalysis (slipped pre-IC4)
Fitted models: 9r96
Deposition Authors: Goovaerts Q
,
Shen J,
Ajjugal Y ,
De Wijngaert B,
Patel SS ,
Das K
Human mitochondrial RNA polymerase structures reveal transcription start site and slippage mechanism.
Shen J,
Goovaerts Q ,
Ajjugal Y ,
De Wijngaert B,
Das K ,
Patel SS
(2025) Mol Cell , 85 , 3137 - 3150.e7
,
Ajjugal Y ,
De Wijngaert B,
Das K ,
Patel SS
(2025) Mol Cell , 85 , 3137 - 3150.e7
Grant Support:
- Research Foundation - Flanders (FWO): 1162825N (Belgium)