EMD-52745

Single-particle
2.89 Å
EMD-52745
3D View Gallery
Deposition: 06/02/2025
Map released: 23/07/2025
Last modified: 23/07/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-52745

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9i8y summary report (pdf.gz) Map-9i8y FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

SpCas12Cas12f1 in complex with sgRNA and cognate DNA

EMD-52745

Single-particle
2.89 Å
EMD-52745
3D View Gallery
Deposition: 06/02/2025
Map released: 23/07/2025
Last modified: 23/07/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Syntrophomonas palmitatica JCM 14374
Sample: SpCas12f1 in complex with sgRNA and cognate DNA
Fitted models: 9i8y

Deposition Authors: Sasnauskas G , Baltramonaitis M , Karvelis T , Siksnys V
Structural and mechanistic insights into the sequential dsDNA cleavage by SpCas12f1.
Madariaga-Marcos J, Baltramonaitis M , Henkel-Heinecke S, Kauert DJ, Irmisch P , Bigelyte-Stankeviciene G, Silanskas A, Karvelis T , Siksnys V , Sasnauskas G , Seidel R
(2025) Nucleic Acids Res , 53
PUBMED: 40650969
DOI: doi:10.1093/nar/gkaf588
ISSN: 1362-4962
ASTM: NARHAD
Grant Support: