EMD-52487

Single-particle
3.44 Å
EMD-52487
3D View Gallery
Deposition: 08/01/2025
Map released: 05/11/2025
Last modified: 24/12/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-52487

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cryo-EM structure of TRPC6 (extra cellular domain) purified and plunged using MISO (micro-purification)

EMD-52487

Single-particle
3.44 Å
EMD-52487
3D View Gallery
Deposition: 08/01/2025
Map released: 05/11/2025
Last modified: 24/12/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: unidentified
Sample: TRPC6 with C-terminal eGFP (extra cellular domain)

Deposition Authors: De Gieter S , Eluru G, Stroobants A , Shrestha B , Erbel P , Efremov RG
MISO: microfluidic protein isolation enables single-particle cryo-EM structure determination from a single cell colony.
Eluru G, De Gieter S , Schenck S , Stroobants A , Shrestha B , Erbel P , Brunner JD , Efremov RG
(2025) Nat Methods , 22 , 2563 - 2573
PUBMED: 41233542
DOI: doi:10.1038/s41592-025-02894-x
ISSN: 1548-7105