EMD-5215

Single-particle
33.0 Å
EMD-5215
3D View Gallery
Deposition: 21/07/2010
Map released: 12/08/2010
Last modified: 02/11/2010
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-5215
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Anthrax toxin PA63 in complex with 1G3

EMD-5215

Single-particle
33.0 Å
EMD-5215
3D View Gallery
Deposition: 21/07/2010
Map released: 12/08/2010
Last modified: 02/11/2010
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 0.5 mg/mL
Buffer
pH: 9.0
Details: 150mM NaCl, 50mM CHES
Staining
Type: NEGATIVE
Details: Holey grids with samples negatively stained across holes with 1% uranyl acetate
Grid
Details: Quantifoil R1.2/1.3
Vitrification
Cryogen name: NONE
Instrument: OTHER
Microscopy [1]
Microscope: FEI TECNAI 12
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 120 kV
Nominal CS: 2 mm
Nominal defocus: 2.0 µm - 2.0 µm
Nominal magnification: 52000.0
Specimen holder model: OTHER
Specimen holder details: Single-tilt
Alignment procedure: LEGACY (Astigmatism: objective lens astigmatism was corrected at 97000 times magnification, Electron beam tilt params: )
Minimum tilt angle: 0
Maximum tilt angle: 45
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: NIKON SUPER COOLSCAN 9000
Sampling interval: 13 µm
Number of real images: 40
Average electron dose per image: 10 e/Å2
Bits per pixel: 8.0
Image processing [1]
Details: The particles were manually selected.
Final reconstruction
Resolution: 33.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Number of images used: 3459
Algorithm: OTHER
Details:
⌯ Applied Symmetry
Point group: C2
Software [1]
Name Version Details
XMIPP - -
Final 2D classification
Number of classes: 20
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Anthrax toxin component PA63 in complex with neutralizing antibody 1G3
Details: ::::EMDATABANK.org::::EMD-5215::::
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 80 80 80
Dimensions (Å) 400 400 400
Voxel size (Å) 5 5 5
Contour list
Primary Level Source
True 0.0416 AUTHOR