EMD-51863

Single-particle
3.9 Å
EMD-51863
3D View Gallery
Deposition: 20/10/2024
Map released: 01/04/2026
Last modified: 27/05/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-51863

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9h4l summary report (pdf.gz) Map-9h4l FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

dCas9 bound to off-target 2 EMX1-1 (-)SC DNA minicircle

EMD-51863

Single-particle
3.9 Å
EMD-51863
3D View Gallery
Deposition: 20/10/2024
Map released: 01/04/2026
Last modified: 27/05/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Streptococcus pyogenes
Sample: dCas9 EMX1-1 on-target complex on a negatively supercoiled DNA minicircle
Fitted models: 9h4l

Deposition Authors: Smith QM , Rueda D
Structural basis of supercoiling-induced CRISPR-Cas9 off-target activity.
Smith QM , Whittle S , Aramayo RJ, Rollins DE, Jalal ASB, Egharevba DI , Morris KL , Pyne ALB , Rueda DS
(2026) Nature , 653 , 627 - 635
PUBMED: 41882360
DOI: doi:10.1038/s41586-026-10255-7
ISSN: 1476-4687
ASTM: NATUAS