EMD-51727

Single-particle
3.4 Å
EMD-51727
3D View Gallery
Deposition: 04/10/2024
Map released: 30/07/2025
Last modified: 03/09/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-51727

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9gzm summary report (pdf.gz) Map-9gzm FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cryo-EM structure of the human mitochondrial RNA polymerase transcription initiation complex (POLRMT/TFAM/TFB2M/DNA/RNA) with a 2-mer RNA (pppGpA) and GTP poised for catalysis (pre-IC3)

EMD-51727

Single-particle
3.4 Å
EMD-51727
3D View Gallery
Deposition: 04/10/2024
Map released: 30/07/2025
Last modified: 03/09/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Homo sapiens, synthetic construct
Sample: Cryo-EM structure of the human mitochondrial RNA polymerase transcription initiation complex (POLRMT/TFAM/TFB2M/DNA/RNA) with a 2-mer RNA (pppGpA) and GTP poised for catalysis (pre-IC3)
Fitted models: 9gzm

Deposition Authors: Goovaerts Q , Shen J, Ajjugal Y , De Wijngaert B, Patel SS , Das K
Human mitochondrial RNA polymerase structures reveal transcription start site and slippage mechanism.
Shen J, Goovaerts Q , Ajjugal Y , De Wijngaert B, Das K , Patel SS
(2025) Mol Cell , 85 , 3137 - 3150.e7
PUBMED: 40712586
DOI: doi:10.1016/j.molcel.2025.07.002
ISSN: 1097-2765
ASTM: MOCEFL