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EMD-51365
Cryo-EM structure of human SLC45A4 in lipid nanodiscs
EMD-51365
Single-particle3.25 Å
Deposition: 16/08/2024Map released: 30/07/2025
Last modified: 22/10/2025
Sample Organism:
Homo sapiens
Sample: Human SLC45A4
Fitted models: 9ghz
Deposition Authors: Markusson S, Newstead S
Sample: Human SLC45A4
Fitted models: 9ghz
Deposition Authors: Markusson S, Newstead S
SLC45A4 is a pain gene encoding a neuronal polyamine transporter.
Middleton SJ 
,
Markusson S,
Akerlund M,
Deme JC ,
Tseng M,
Li W,
Zuberi SR,
Kuteyi G,
Sarkies P,
Baskozos G ,
Perez-Sanchez J ,
Farah A,
Hebert HL,
Toikumo S,
Yu Z,
Maxwell S ,
Dong YY,
Kessler BM ,
Kranzler HR ,
Linley JE,
Smith BH ,
Lea SM ,
Parker JL ,
Lyssenko V,
Newstead S,
Bennett DL
(2025) Nature , 646 , 404 - 412
,
Markusson S,
Akerlund M,
Deme JC ,
Tseng M,
Li W,
Zuberi SR,
Kuteyi G,
Sarkies P,
Baskozos G ,
Perez-Sanchez J ,
Farah A,
Hebert HL,
Toikumo S,
Yu Z,
Maxwell S ,
Dong YY,
Kessler BM ,
Kranzler HR ,
Linley JE,
Smith BH ,
Lea SM ,
Parker JL ,
Lyssenko V,
Newstead S,
Bennett DL
(2025) Nature , 646 , 404 - 412
Secondary citations:
- Afonine PV, Poon BK, Read RJ, Sobolev OV, Terwilliger TC, Urzhumtsev A & Adams PD. (2018) Real-space refinement in PHENIX for cryo-EM and crystallography. Acta Crystallogr D Struct Biol, 74, 531 - 544
- Emsley P, Lohkamp B, Scott WG & Cowtan K. (2010) Features and development of Coot. Acta Crystallogr D Struct Biol, 66, 486 - 501
- & Croll TI. (2018) ISOLDE: a physically realistic environment for model building into low-resolution electron-density maps. Acta Crystallogr D Struct Biol, 74, 519 - 530
- Punjani A, Rubinstein JL, Fleet DJ & Brubaker MA. (2017) cryoSPARC: algorithms for rapid unsupervised cryo-EM structure determination. 14, 290 - 296
- Zivanov J, Nakane T, Forsberg BO, Kimanius D, Hagen WJ, Lindahl E & Scheres SH. (2018) New tools for automated high-resolution cryo-EM structure determination in RELION-3. eLife, 7,
- Caesar J, Reboul CF, Machello C, Kiesewetter S, Tang ML, Deme JC, Johnson S, Elmlund D, Lea SM & Elmlund H. (2020) SIMPLE 3.0. Stream single-particle cryo-EM analysis in real time. J Struct Biol, 212, 107635
Grant Support:
- Wellcome Trust: 215519/Z/19/Z (United Kingdom)
- Wellcome Trust: 219531/Z/19/Z (United Kingdom)