EMD-50510

Single-particle
3.43 Å
EMD-50510
3D View Gallery
Deposition: 31/05/2024
Map released: 07/05/2025
Last modified: 07/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-50510

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9fjr summary report (pdf.gz) Map-9fjr FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cryo-EM structure of Mycobacterium tuberculosis sigma-B RNA polymerase bound to -10 promoter element ssDNA oligo - sigma-B docked conformation

EMD-50510

Single-particle
3.43 Å
EMD-50510
3D View Gallery
Deposition: 31/05/2024
Map released: 07/05/2025
Last modified: 07/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Mycobacterium tuberculosis H37Rv, Escherichia coli
Sample: RNA polymerase holoenzyme bound to -10 promoter element ssDNA oligo - sigB docked state
Fitted models: 9fjr

Deposition Authors: Brodolin K , Blaise M
Single-stranded DNA drives sigma subunit loading onto mycobacterial RNA polymerase to unlock initiation-competent conformations.
Vishwakarma RK, Marechal N, Morichaud Z, Blaise M , Margeat E, Brodolin K
(2025) Nucleic Acids Res , 53
PUBMED: 40240004
DOI: doi:10.1093/nar/gkaf272
ISSN: 1362-4962
ASTM: NARHAD