EMD-5027

Helical reconstruction
28.0 Å
EMD-5027
3D View Gallery
Deposition: 29/08/2008
Map released: 05/05/2009
Last modified: 02/04/2014
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-5027
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 3edl summary report (pdf.gz) Map and 3edl FULL report (pdf.gz)
EMICSS annotations (xml)

Kinesin13-Microtubule Ring Complex

EMD-5027

Helical reconstruction
28.0 Å
EMD-5027
3D View Gallery
Deposition: 29/08/2008
Map released: 05/05/2009
Last modified: 02/04/2014
Overview 3D View Sample Experiment Validation Additional data Links
Method: Helical reconstruction
Aggregation State: Filament
Specimen preparation [1]
Concentration: 0.6 mg/mL
Buffer
pH: 6.8
Details: 80 mM Pipes, pH 6.8, 2 mM MgCl2, 1 mM EGTA, 2 mM AMP-PNP, 0.02 mM taxol
Grid
Details: Quantifoil grids
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Instrument: OTHER
Method: Blot for 2 seconds, Blot Offset (mm) -2, Plunge Time 1 sec, Wait Time 0 sec
Details: Vitrification instrument: VitroBot
Microscopy [2]
Microscope: FEI TECNAI 20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: LAB6
Acceleration voltage: 120 kV
Nominal defocus: 1.5 µm - 2.5 µm
Nominal magnification: 50000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Side entry liquid nitrogen-cooled cryo specimen holder
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: OTHER
Sampling interval: 12.5 µm
Number of real images: 5
Average electron dose per image: 9 e/Å2
Bits per pixel: 16.0
Microscope: FEI TECNAI F20
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 200 kV
Nominal magnification: 50000.0
Specimen holder model: GATAN LIQUID NITROGEN
Specimen holder details: Side entry liquid nitrogen-cooled cryo specimen holder
Image Recording [1]
Detector category: FILM
Detector model: KODAK SO-163 FILM
Scanner: OTHER
Sampling interval: 12.5 µm
Number of real images: 5
Average electron dose per image: 9 e/Å2
Bits per pixel: 16.0
Image processing [1]
Final reconstruction
Resolution: 28.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Algorithm: OTHER
Software [1]
Name Version Details
MRC, SUPRIM, Phoelix, Other - -
CTF correction
Details: Each Particle
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Kinesin 13-Microtubule Ring Complex
Details: ::::EMDATABANK.org::::EMD-5027::::
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 101 101 41
Dimensions (Å) 606.0 606.0 246.0
Voxel size (Å) 6.0 6.0 6.0
Contour list
Primary Level Source
True 11.1 AUTHOR