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EMD-49594

Single-particle
2.13 Å
EMD-49594
3D View Gallery
Deposition: 08/03/2025
Map released: 18/06/2025
Last modified: 02/07/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-49594
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 9no7 summary report (pdf.gz) Map and 9no7 FULL report (pdf.gz)
EMICSS annotations (xml)

Cryo-EM structure of the wild-type Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys at 2.13A resolution

EMD-49594

Single-particle
2.13 Å
EMD-49594
3D View Gallery
Deposition: 08/03/2025
Map released: 18/06/2025
Last modified: 02/07/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Thermus thermophilus HB8
Sample: Thermus thermophilus 70S ribosome in complex with mRNA, A-site Q230-N5-methylated Release Factor 1, and P-site 2'-deoxy-A76-fMEAAAKC-peptidyl-tRNAcys
Fitted models: 9no7

Deposition Authors: Aleksandrova EV , Syroegin EA , Basu RS , Vassilevski AA , Gagnon MG , Polikanov YS
Mechanism of release factor-mediated peptidyl-tRNA hydrolysis on the ribosome.
Aleksandrova EV , Syroegin EA , Basu RS , Vassilevski AA , Gagnon MG , Polikanov YS
(2025) Science , 388 , eads9030 - eads9030
PUBMED: 40536958
DOI: doi:10.1126/science.ads9030
ISSN: 1095-9203
ASTM: SCIEAS