EMD-4811

Single-particle
3.04 Å
EMD-4811
3D View Gallery
Deposition: 12/04/2019
Map released: 03/07/2019
Last modified: 22/05/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-4811
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6rda summary report (pdf.gz) Map and 6rda FULL report (pdf.gz)
EMICSS annotations (xml)

CryoEM structure of Polytomella F-ATP synthase, Primary rotary state 1, monomer-masked refinement

EMD-4811

Single-particle
3.04 Å
EMD-4811
3D View Gallery
Deposition: 12/04/2019
Map released: 03/07/2019
Last modified: 22/05/2024
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Concentration: 3.5 mg/mL
Buffer
pH: 7.8
Vitrification
Cryogen name: ETHANE
Chamber humidity: 100%
Chamber temperature: 277 K
Instrument: FEI VITROBOT MARK IV
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
C2 aperture diameter: 70.0 µm
Nominal CS: 2.7 mm
Nominal defocus: -0.4 µm - -5.0 µm
Nominal magnification: 75000.0
Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Cooling holder cryogen: NITROGEN
Alignment procedure: BASIC
Image Recording [1]
Detector model: FEI FALCON III (4k x 4k)
Detector mode: COUNTING
Dimensions: 4096 pixel x 4096 pixel
Average electron dose per image: 35.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.04 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 400918
Software [1]
Name Version Details
RELION 3 -
Startup model [1]
Type: OTHER
⦨ Initial angle assignment
Type: NOT APPLICABLE
⦩ Final angle assignment
Type: NOT APPLICABLE
Particle selection [1]
Selected Ref. model Method Software Details
735197 - - - -
Final 3D classification
Software [1]
Name Version Details
RELION 3 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 480 480 480
Dimensions (Å) 505.44 505.44 505.44
Voxel size (Å) 1.053 1.053 1.053
Contour list
Primary Level Source
True 0.04 AUTHOR