EMD-47932

Single-particle
3.23 Å
EMD-47932
3D View Gallery
Deposition: 15/11/2024
Map released: 10/09/2025
Last modified: 19/11/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-47932
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 9ed4 summary report (pdf.gz) Map and 9ed4 FULL report (pdf.gz)
EMICSS annotations (xml)

A composite map of mTORC1-Rag-Ragultor-4EBP1 on membrane

EMD-47932

Single-particle
3.23 Å
EMD-47932
3D View Gallery
Deposition: 15/11/2024
Map released: 10/09/2025
Last modified: 19/11/2025
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: TFS KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Nominal defocus: 0.9 µm - 2.0 µm
Image Recording [1]
Detector model: GATAN K3 BIOQUANTUM (6k x 4k)
Average electron dose per image: 30.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.23 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 359012
Software [1]
Name Version Details
CryoSPARC 4.4 -
Startup model [1]
Type: INSILICO MODEL
Insilico model: Ab-initio reconstruction by cryoSPARC
⦨ Initial angle assignment
Type: MAXIMUM LIKELIHOOD
⦩ Final angle assignment
Type: MAXIMUM LIKELIHOOD
CTF correction
Type: PHASE FLIPPING AND AMPLITUDE CORRECTION
Software [1]
Name Version Details
CryoSPARC 3.2 -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: A composite map of mLST8-mTOR-Rheb and Raptor-Rag-Ragulator subcomplexes
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 448 448 448
Dimensions (Å) 465.91998 465.91998 465.91998
Voxel size (Å) 1.04 1.04 1.04
Contour list
Primary Level Source
True 0.06 AUTHOR