EMD-47853

Tomography
EMD-47853
Gallery
Deposition: 11/11/2024
Map released: 18/03/2026
Last modified: 18/03/2026
Overview Sample Experiment Validation Additional data Links
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EMD-47853
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Montaged cryo-ET map of individual particle 3D reconstructions of ~2 kb negatively supercoiled plasmids (delta Lk centered on -15) under near physiological salt condition (40 mM K+, 5 mM Mg2+) in the presence of transcribed RNA polymerase and dCas9

EMD-47853

Tomography
EMD-47853
Gallery
Deposition: 11/11/2024
Map released: 18/03/2026
Last modified: 18/03/2026
Overview Sample Experiment Validation Additional data Links
Sample Organism: Escherichia coli
Sample: 2 kb negatively supercoiled pUC19 plasmids containing a T7A1-U promoter in the presence of transcribed RNA polymerase and dCas9

Deposition Authors: Zhang M , Bustamante C

Interplay Between DNA Supercoiling and RNA Polymerase Localization at Plectoneme Apices Modulate Transcription Dynamics
Zhang M , Bustamante C
To Be Published