EMD-47850

Tomography
EMD-47850
Gallery
Deposition: 11/11/2024
Map released: 18/03/2026
Last modified: 18/03/2026
Overview Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-47850
Download
3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
EMICSS annotations (xml)

Montaged cryo-ET map of individual particle 3D reconstructions of ~2 kb negatively supercoiled plasmids (delta Lk centered on -15) under near physiological salt condition (40 mM K+, 5 mM Mg2+) with RNA polymerase bound during active transcription

EMD-47850

Tomography
EMD-47850
Gallery
Deposition: 11/11/2024
Map released: 18/03/2026
Last modified: 18/03/2026
Overview Sample Experiment Validation Additional data Links
Sample Organism: Escherichia coli
Sample: 2 kb negatively supercoiled pUC19 plasmids containing a T7A1-U promoter with RNA polymerase bound during active transcription

Deposition Authors: Zhang M , Bustamante C

Interplay Between DNA Supercoiling and RNA Polymerase Localization at Plectoneme Apices Modulate Transcription Dynamics
Zhang M , Bustamante C
To Be Published