EMD-44859

Single-particle
2.6 Å
EMD-44859
3D View Gallery
Deposition: 13/05/2024
Map released: 28/05/2025
Last modified: 29/04/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-44859

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9bs6 summary report (pdf.gz) Map-9bs6 FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

CryoEM structure of ThermoCas9 in post-cleavage state with a DNA containing NNNNCGA PAM

EMD-44859

Single-particle
2.6 Å
EMD-44859
3D View Gallery
Deposition: 13/05/2024
Map released: 28/05/2025
Last modified: 29/04/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Geobacillus thermodenitrificans
Sample: Ternary complex of ThermoCas9 bound with RNA and DNA containing NNNNCGA PAM
Fitted models: 9bs6

Deposition Authors: Zhao Y, Shu Y
Molecular basis for methylation-sensitive editing by Cas9.
Roth MO, Shu Y, Zhao Y, Trasanidou D, Hoffman RD, Sudfeld C, Bouzetos E, Trasanidis N, Zawrotny M, Gelasco MK, Medina ML, Das A, Rai J, Goswami HN, Wang B, van der Oost J, Li H
(2026) Nature
PUBMED: 41986708
DOI: doi:10.1038/s41586-026-10384-z
ISSN: 1476-4687
ASTM: NATUAS