EMD-43769

Single-particle
2.2 Å
EMD-43769
3D View Gallery
Deposition: 22/02/2024
Map released: 26/02/2025
Last modified: 15/04/2026
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-43769

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-9ar4 summary report (pdf.gz) Map-9ar4 FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

CryoEM structure of ThermoCas9 in post-cleavage state bound with the DNA containing NNNNCCA PAM

EMD-43769

Single-particle
2.2 Å
EMD-43769
3D View Gallery
Deposition: 22/02/2024
Map released: 26/02/2025
Last modified: 15/04/2026
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: synthetic construct, Geobacillus thermodenitrificans
Sample: ThermoCas9 Post-cleavage complex
Fitted models: 9ar4

Deposition Authors: Shu Y, Zhao Y, Li H
Molecular basis for methylation-sensitive editing by Cas9
Roth MO, Shu Y, Zhao Y, Trasanidou D, Hoffman RD, Trasanidis N, Gelasco MK, Medina ML, Das D, Rai J, Wang B, Li H
(2026) Nature
DOI: doi:10.1038/s41586-026-10384-z
ISSN: 1476-4687
ASTM: NATUAS