EMD-4341

Helical reconstruction
3.93 Å
EMD-4341
3D View Gallery
Deposition: 22/03/2018
Map released: 21/11/2018
Last modified: 15/05/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-4341
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 6g1x summary report (pdf.gz) Map and 6g1x FULL report (pdf.gz)
EMICSS annotations (xml)

CryoEM structure of the MDA5-dsRNA filament with 91-degree helical twist

EMD-4341

Helical reconstruction
3.93 Å
EMD-4341
3D View Gallery
Deposition: 22/03/2018
Map released: 21/11/2018
Last modified: 15/05/2024
Overview 3D View Sample Experiment Validation Additional data Links
Name: MDA5-dsRNA helical filament
Complex [3]
Name: MDA5-dsRNA helical filament
Molecular weight
Experimental Theoretical Method
- 2.22 kDa/nm -
Name: MDA5
Details: DExD/H-box helicase consisting of Hel1, Hel2, Hel2i, and pincer domains, followed by a C-terminal domain
Natural source [1]
Organism: Mus musculus
Name: Double-stranded RNA from bacteriophage Phi6
Natural source [1]
Organism: Pseudomonas phage phi6
Protein [1]
LEVO
Name: Interferon-induced helicase C domain-containing protein 1
EC number: 3.6.4.13
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Mus musculus
Molecular weight
Experimental Theoretical Method
- 80 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - - -
RNA [2]
Name: RNA (5'-R(P*UP*CP*CP*AP*UP*GP*CP*GP*CP*AP*UP*GP*AP*CP*G)-3')
Number of copies: 1
Natural source
Organism: Pseudomonas phage phi6
Molecular weight
Experimental Theoretical Method
- 4 kDa -
Name: RNA (5'-R(P*CP*GP*UP*CP*AP*UP*GP*CP*GP*CP*AP*UP*GP*GP*A)-3')
Number of copies: 1
Natural source
Organism: Pseudomonas phage phi6
Molecular weight
Experimental Theoretical Method
- 4 kDa -
Ligand [1]
Name: ZINC ION
HET code: ZN
Number of copies: 1
ChEMBL ChEBI DrugBank
Molecular weight
Experimental Theoretical Method
- 65 Da -