EMD-4304

Subtomogram averaging
33.0 Å
EMD-4304
3D View Gallery
Deposition: 21/02/2018
Map released: 31/10/2018
Last modified: 07/11/2018
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-4304
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3D volume (map.gz) Experimental metadata (xml)
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Cryo-EM subtomogram average of IFT complex A in anterograde IFT trains (Chlamydomonas reinhardtii)

EMD-4304

Subtomogram averaging
33.0 Å
EMD-4304
3D View Gallery
Deposition: 21/02/2018
Map released: 31/10/2018
Last modified: 07/11/2018
Overview 3D View Sample Experiment Validation Additional data Links
Method: Subtomogram averaging
Aggregation State: 2D Array
Specimen preparation [1]
Buffer
pH: 7.0
Grid
Model: Quantifoil R3.5/1
Pretreatment
Type: GLOW DISCHARGE
Atmosphere:
Support Film [1]
Material Topology Thickness
CARBON HOLEY -
Vitrification
Cryogen name: ETHANE
Instrument: LEICA EM GP
Microscopy [1]
Microscope: FEI TITAN
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Specialist optics
Energy filter
Name: GIF Quantum LS
Image Recording [1]
Detector model: GATAN K2 SUMMIT (4k x 4k)
Detector mode: SUPER-RESOLUTION
Average electron dose per image: 2.1 e/Å2
Image processing [1]
Final reconstruction
Resolution: 33.0 Å ( BY AUTHOR)
Resolution method: FSC 0.5 CUT-OFF
Software [1]
Name Version Details
PEET 1.11.0 -
⦩ Final angle assignment
Type: NOT APPLICABLE
Extraction
Number of images used: 750
CTF correction
Software [1]
Name Version Details
CTFPHASEFLIP - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotation details: Cryo-EM subtomogram average of IFT complex A in anterograde IFT trains (Chlamydomonas reinhartdii)
⬡ Geometry
X Y Z
Origin -8 -10 -7
Dimensions (px) 22 17 33
Dimensions (Å) 310.86002 240.21 466.29
Voxel size (Å) 14.130001 14.13 14.13
Contour list
Primary Level Source
True 0.512 AUTHOR