EMD-42837

Single-particle
3.17 Å
EMD-42837
3D View Gallery
Deposition: 14/11/2023
Map released: 29/05/2024
Last modified: 03/07/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-42837

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-8uza summary report (pdf.gz) Map-8uza FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

Cryo-EM structure of GeoCas9 in complex with sgRNA and target DNA

EMD-42837

Single-particle
3.17 Å
EMD-42837
3D View Gallery
Deposition: 14/11/2023
Map released: 29/05/2024
Last modified: 03/07/2024
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Geobacillus stearothermophilus, Mus musculus
Sample: Ternary complex of deactivated wild type GeoCas9 with sgRNA and target DNA
Fitted models: 8uza

Deposition Authors: Eggers AR, Soczek KM, Tuck OT, Doudna JA
Rapid DNA unwinding accelerates genome editing by engineered CRISPR-Cas9.
Eggers AR, Chen K, Soczek KM, Tuck OT, Doherty EE, Xu B, Trinidad MI, Thornton BW, Yoon PH, Doudna JA
(2024) Cell , 187 , 3249
PUBMED: 38781968
DOI: doi:10.1016/j.cell.2024.04.031
ISSN: 1097-4172