EMD-4117

Helical reconstruction
3.8 Å
EMD-4117
3D View Gallery
Deposition: 23/09/2016
Map released: 18/01/2017
Last modified: 18/01/2017
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-4117
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3D volume (map.gz) Experimental metadata (xml)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 5ly4 summary report (pdf.gz) Map and 5ly4 FULL report (pdf.gz)
EMICSS annotations (xml)

cryoEM structure of crenactin double helical filament at 3.8A resolution

EMD-4117

Helical reconstruction
3.8 Å
EMD-4117
3D View Gallery
Deposition: 23/09/2016
Map released: 18/01/2017
Last modified: 18/01/2017
Overview 3D View Sample Experiment Validation Additional data Links
Method: Helical reconstruction
Aggregation State: Filament
Specimen preparation [1]
Buffer
pH: 7.0
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI POLARA 300
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: FEI FALCON II (4k x 4k)
Detector mode: INTEGRATING
Number of real images: 1474
Average electron dose per image: 40.0 e/Å2
Image processing [1]
Details: Falcon III prototype at 46 frames per second
Final reconstruction
Resolution: 3.8 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 470396
Details: Helical segments divided at the filament level into the two half sets at the beginning.
⌯ Applied Symmetry
ΔΖ: 25.6 Å
ΔΦ: -161.9°
Axial symmetry: C1
Software [1]
Name Version Details
RELION 2.0 -
Startup model [1]
Type: PDB ENTRY
PDB model: 4CJ7
⦩ Final angle assignment
Type: NOT APPLICABLE
CTF correction
Software [1]
Name Version Details
GCTF - -
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
⬡ Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 280 280 280
Dimensions (Å) 375.2 375.2 375.2
Voxel size (Å) 1.34 1.34 1.34
Contour list
Primary Level Source
True 0.128 AUTHOR