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EMD-41074

Single-particle
3.15 Å
EMD-41074
3D View Gallery
Deposition: 16/06/2023
Map released: 01/05/2024
Last modified: 14/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-41074
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 8t6p summary report (pdf.gz) Map and 8t6p FULL report (pdf.gz)
EMICSS annotations (xml)

SpRY-Cas9:gRNA complex targeting TAC PAM DNA with 2 bp R-loop

EMD-41074

Single-particle
3.15 Å
EMD-41074
3D View Gallery
Deposition: 16/06/2023
Map released: 01/05/2024
Last modified: 14/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Streptococcus pyogenes, Escherichia phage Lambda
Sample: Ternary complex of SpRY-Cas9 with gRNA and NAC PAM DNA after one minute of DNA incubation with 2 bp R-loop
Fitted models: 8t6p

Deposition Authors: Hibshman GN , Bravo JPK, Taylor DW
Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9.
Hibshman GN , Bravo JPK, Hooper MM , Dangerfield TL, Zhang H, Finkelstein IJ , Johnson KA, Taylor DW
(2024) Nat Commun , 15 , 3663 - 3663
PUBMED: 38688943
DOI: doi:10.1038/s41467-024-47830-3
ISSN: 2041-1723