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EMD-40931

Single-particle
3.79 Å
EMD-40931
3D View Gallery
Deposition: 31/05/2023
Map released: 02/04/2025
Last modified: 28/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-40931
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 8t02 summary report (pdf.gz) Map and 8t02 FULL report (pdf.gz)
EMICSS annotations (xml)

Reconstituted E. coli RNA polymerase post-termination complex on negatively-supercoiled DNA: unwinding duplex DNA (rPTCi)

EMD-40931

Single-particle
3.79 Å
EMD-40931
3D View Gallery
Deposition: 31/05/2023
Map released: 02/04/2025
Last modified: 28/05/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Escherichia coli, synthetic construct
Sample: E. coli Post-termination complex with open DNA bubble on negatively supercoiled DNA
Fitted models: 8t02

Deposition Authors: Brewer JJ, Darst SA , Campbell EA
RapA opens the RNA polymerase clamp to disrupt post-termination complexes and prevent cytotoxic R-loop formation.
Brewer JJ, Inlow K , Mooney RA, Bosch B, Olinares PDB, Marcelino LP , Chait BT , Landick R , Gelles J , Campbell EA , Darst SA
(2025) Nat Struct Mol Biol , 32 , 639 - 649
PUBMED: 39779919
DOI: doi:10.1038/s41594-024-01447-8
ISSN: 1545-9985