EMD-40681

Single-particle
3.26 Å
EMD-40681
3D View Gallery
Deposition: 03/05/2023
Map released: 01/05/2024
Last modified: 15/05/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-40681

Archive Files (Depositor)

Primary 3D volume (map.gz) Half-map 1 Half-map 2 (map.gz) Experimental metadata (xml) Experimental metadata (cif.gz)

Validation (wwPDB)

Map-only validation report (pdf.gz) Map-8spq summary report (pdf.gz) Map-8spq FULL report (pdf.gz)

EMDB Annotations

EMICSS entry mapping (xml)

EMDB Files

VA raw map (map) VA resolution mask (mrc)

SpRY-Cas9:gRNA complex targeting TGG PAM DNA

EMD-40681

Single-particle
3.26 Å
EMD-40681
3D View Gallery
Deposition: 03/05/2023
Map released: 01/05/2024
Last modified: 15/05/2024
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Escherichia phage Lambda, Streptococcus pyogenes, Streptococcus pyogenes serotype M1
Sample: Ternary complex of SpRY-Cas9 with lambda 1 gRNA and lambda 1 DNA
Fitted models: 8spq

Deposition Authors: Hibshman GN , Bravo JPK, Taylor DW
Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9.
Hibshman GN , Bravo JPK, Hooper MM , Dangerfield TL, Zhang H, Finkelstein IJ , Johnson KA, Taylor DW
(2024) Nat Commun , 15 , 3663 - 3663
PUBMED: 38688943
DOI: doi:10.1038/s41467-024-47830-3
ISSN: 2041-1723