EMD-3941

Single-particle
3.0 Å
EMD-3941 Deposition: 18/10/2017
Map released: 18/04/2018
Last modified: 13/11/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-3941

Structure of the chloroplast ribosome with chl-RRF and hibernation-promoting factor

EMD-3941

Single-particle
3.0 Å
EMD-3941 Deposition: 18/10/2017
Map released: 18/04/2018
Last modified: 13/11/2024
Overview 3D View Sample Experiment Validation Additional data Links
Method: Single-particle
Aggregation State: Particle
Specimen preparation [1]
Buffer
pH: 7.4
Vitrification
Cryogen name: ETHANE
Microscopy [1]
Microscope: FEI TITAN KRIOS
Illumination mode: FLOOD BEAM
Imaging mode: BRIGHT FIELD
Electron source: FIELD EMISSION GUN
Acceleration voltage: 300 kV
Image Recording [1]
Detector model: GATAN K2 QUANTUM (4k x 4k)
Detector mode: COUNTING
Average electron dose per image: 4.0 e/Å2
Image processing [1]
Final reconstruction
Resolution: 3.0 Å ( BY AUTHOR)
Resolution method: FSC 0.143 CUT-OFF
Number of images used: 130300
Applied Symmetry
Point group: C1
Startup model [1]
Type: EMDB MAP
Initial angle assignment
Final angle assignment
Map
Format: CCP4
Data type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Geometry
X Y Z
Origin 0 0 0
Dimensions (px) 420 420 420
Dimensions (Å) 445.19998 445.19998 445.19998
Voxel size (Å) 1.06 1.06 1.06
Contour list
Primary Level Source
True 0.03 EMDB