EMD-3460

Single-particle
3.8 Å
EMD-3460
3D View Gallery
Deposition: 08/11/2016
Map released: 11/01/2017
Last modified: 15/05/2024
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-3460
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3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
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EMICSS annotations (xml)

Cryo-EM structure of Lambda Phage protein GamS bound to RecBCD

EMD-3460

Single-particle
3.8 Å
EMD-3460
3D View Gallery
Deposition: 08/11/2016
Map released: 11/01/2017
Last modified: 15/05/2024
Overview 3D View Sample Experiment Validation Additional data Links
Name: RecBCD complex bound to inhibitory protein lambda GamS
Complex [3]
Name: RecBCD complex bound to inhibitory protein lambda GamS
Details: RecBCD and GamS were expressed and purified separately. They were mixed with an excess of GamS then passed through a size exclusion column to separate out free GamS.
Molecular weight
Experimental Theoretical Method
- 350 kDa -
Complex Portal [1]
ID Name Overlap
CPX-2197 Exodeoxyribonuclease V complex 0.75
Name: RecBCD helicase/nuclease complex
Natural source [1]
Organism: Escherichia coli
Molecular weight
Experimental Theoretical Method
- 330 kDa -
Complex Portal [1]
ID Name Overlap
CPX-2197 Exodeoxyribonuclease V complex 1.00
Name: Lambda GamS
Natural source [1]
Organism: Enterobacteria phage lambda
Molecular weight
Experimental Theoretical Method
- 20 kDa -
Protein [4]
LEVO
Name: RecBCD enzyme subunit RecB
EC number: 3.1.11.5
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Escherichia coli
Molecular weight
Experimental Theoretical Method
- 134 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - - -
LEVO
Name: RecBCD enzyme subunit RecC
EC number: 3.1.11.5
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Escherichia coli
Molecular weight
Experimental Theoretical Method
- 128 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - - -
LEVO
Name: RecBCD enzyme subunit RecD
EC number: 3.1.11.5
Number of copies: 1
UniProtKB PDBe-KB AlphaFold DB
Domains
Natural source
Organism: Escherichia coli
Molecular weight
Experimental Theoretical Method
- 67 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - - -
LEVO
Name: Host-nuclease inhibitor protein gam
Number of copies: 2
Details: GamL is cleaved at position Y44 when expressed in E.coli into GamS (see paper - pubmed id 17544443). We had the GamS gene synthesised to start at M41 for this project. It purifies as a dimer.
UniProtKB PDBe-KB
Domains
Natural source
Organism: Enterobacteria phage lambda
Molecular weight
Experimental Theoretical Method
- 11 kDa -
Recombinant Expression
Organism Strain Cell Plasmid
Escherichia coli BL21(DE3) - - -