Happy Holidays and New year from the EMDB! Please be advised that the EMDB team will be operating at reduced capacity between December 15th - January 12th. With this in mind we would ask that you be prepared for longer reply times through both our helpdesk system and the OneDep deposition system. well done on a productive 2025, wishing you all the best and we will see you in 2026!

EMD-34028

Single-particle
3.38 Å
EMD-34028
3D View Gallery
Deposition: 07/08/2022
Map released: 17/08/2022
Last modified: 02/07/2025
Overview 3D View Sample Experiment Validation Additional data Links
Overview 3D View Sample Experiment Validation Additional data Links

EMD-34028
Download
3D volume (map.gz) Experimental metadata (xml) Experimental Metadata (cif.gz)
Map-only Validation report (pdf.gz) Map-only Validation report (cif.gz) Map-only Validation report (xml.gz)
Map and PDB model 7yqk summary report (pdf.gz) Map and 7yqk FULL report (pdf.gz)
EMICSS annotations (xml)

cryo-EM structure of gammaH2AXK15ub-H4K20me2 nucleosome bound to 53BP1

EMD-34028

Single-particle
3.38 Å
EMD-34028
3D View Gallery
Deposition: 07/08/2022
Map released: 17/08/2022
Last modified: 02/07/2025
Overview 3D View Sample Experiment Validation Additional data Links
Sample Organism: Homo sapiens, synthetic construct
Sample: Complex of 53BP1(1484-1972) and gammaH2AXK15ub-H4K20me2 nucleosome
Fitted models: 7yqk

Deposition Authors: Ai HS, GuoChao C, Qingyue G, Ze-Bin T, Zhiheng D, Xin L, Fan Y, Ziyu X, Jia-Bin L, Changlin T, Liu L
Chemical Synthesis of Post-Translationally Modified H2AX Reveals Redundancy in Interplay between Histone Phosphorylation, Ubiquitination, and Methylation on the Binding of 53BP1 with Nucleosomes.
Ai H , Chu GC, Gong Q, Tong ZB, Deng Z, Liu X, Yang F, Xu Z, Li JB , Tian C, Liu L
(2022) J Am Chem Soc , 144 , 18329 - 18337
PUBMED: 36166692
DOI: doi:10.1021/jacs.2c06156
ISSN: 1520-5126
ASTM: JACSAT